Generation and analysis of expression sequence tags from haustoria of the wheat stripe rust fungus Puccinia striiformis f. sp. Tritici.
Identifieur interne : 000881 ( Main/Exploration ); précédent : 000880; suivant : 000882Generation and analysis of expression sequence tags from haustoria of the wheat stripe rust fungus Puccinia striiformis f. sp. Tritici.
Auteurs : Chuntao Yin [États-Unis] ; Xianming Chen ; Xiaojie Wang ; Qingmei Han ; Zhensheng Kang ; Scot H. HulbertSource :
- BMC genomics [ 1471-2164 ] ; 2009.
Descripteurs français
- KwdFr :
- MESH :
- génétique : ARN fongique, Basidiomycota.
- microbiologie : Maladies des plantes, Triticum.
- Analyse de profil d'expression de gènes, Analyse de séquence d'ADN, Banque de gènes, Gènes fongiques, Étiquettes de séquences exprimées.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : RNA, Fungal.
- genetics : Basidiomycota.
- microbiology : Plant Diseases, Triticum.
- Expressed Sequence Tags, Gene Expression Profiling, Gene Library, Genes, Fungal, Sequence Analysis, DNA.
Abstract
BACKGROUND
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. In spite of its agricultural importance, the genomics and genetics of the pathogen are poorly characterized. Pst transcripts from urediniospores and germinated urediniospores have been examined previously, but little is known about genes expressed during host infection. Some genes involved in virulence in other rust fungi have been found to be specifically expressed in haustoria. Therefore, the objective of this study was to generate a cDNA library to characterize genes expressed in haustoria of Pst.
RESULTS
A total of 5,126 EST sequences of high quality were generated from haustoria of Pst, from which 287 contigs and 847 singletons were derived. Approximately 10% and 26% of the 1,134 unique sequences were homologous to proteins with known functions and hypothetical proteins, respectively. The remaining 64% of the unique sequences had no significant similarities in GenBank. Fifteen genes were predicted to be proteins secreted from Pst haustoria. Analysis of ten genes, including six secreted protein genes, using quantitative RT-PCR revealed changes in transcript levels in different developmental and infection stages of the pathogen.
CONCLUSIONS
The haustorial cDNA library was useful in identifying genes of the stripe rust fungus expressed during the infection process. From the library, we identified 15 genes encoding putative secreted proteins and six genes induced during the infection process. These genes are candidates for further studies to determine their functions in wheat-Pst interactions.
DOI: 10.1186/1471-2164-10-626
PubMed: 20028560
PubMed Central: PMC2805700
Affiliations:
Links toward previous steps (curation, corpus...)
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Hulbert</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:20028560</idno><idno type="pmid">20028560</idno><idno type="doi">10.1186/1471-2164-10-626</idno><idno type="pmc">PMC2805700</idno><idno type="wicri:Area/Main/Corpus">000847</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000847</idno><idno type="wicri:Area/Main/Curation">000847</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000847</idno><idno type="wicri:Area/Main/Exploration">000847</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Generation and analysis of expression sequence tags from haustoria of the wheat stripe rust fungus Puccinia striiformis f. sp. Tritici.</title><author><name sortKey="Yin, Chuntao" sort="Yin, Chuntao" uniqKey="Yin C" first="Chuntao" last="Yin">Chuntao Yin</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Washington State University, Pullman, 99164-6430, USA. chunto_yin@wsu.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, Washington State University, Pullman, 99164-6430</wicri:regionArea><wicri:noRegion>99164-6430</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Xianming" sort="Chen, Xianming" uniqKey="Chen X" first="Xianming" last="Chen">Xianming Chen</name></author><author><name sortKey="Wang, Xiaojie" sort="Wang, Xiaojie" uniqKey="Wang X" first="Xiaojie" last="Wang">Xiaojie Wang</name></author><author><name sortKey="Han, Qingmei" sort="Han, Qingmei" uniqKey="Han Q" first="Qingmei" last="Han">Qingmei Han</name></author><author><name sortKey="Kang, Zhensheng" sort="Kang, Zhensheng" uniqKey="Kang Z" first="Zhensheng" last="Kang">Zhensheng Kang</name></author><author><name sortKey="Hulbert, Scot H" sort="Hulbert, Scot H" uniqKey="Hulbert S" first="Scot H" last="Hulbert">Scot H. Hulbert</name></author></analytic><series><title level="j">BMC genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term><term>Expressed Sequence Tags (MeSH)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Library (MeSH)</term><term>Genes, Fungal (MeSH)</term><term>Plant Diseases (microbiology)</term><term>RNA, Fungal (genetics)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Triticum (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN fongique (génétique)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Banque de gènes (MeSH)</term><term>Basidiomycota (génétique)</term><term>Gènes fongiques (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Triticum (microbiologie)</term><term>Étiquettes de séquences exprimées (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Fungal</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN fongique</term><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Expressed Sequence Tags</term><term>Gene Expression Profiling</term><term>Gene Library</term><term>Genes, Fungal</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Analyse de séquence d'ADN</term><term>Banque de gènes</term><term>Gènes fongiques</term><term>Étiquettes de séquences exprimées</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. In spite of its agricultural importance, the genomics and genetics of the pathogen are poorly characterized. Pst transcripts from urediniospores and germinated urediniospores have been examined previously, but little is known about genes expressed during host infection. Some genes involved in virulence in other rust fungi have been found to be specifically expressed in haustoria. Therefore, the objective of this study was to generate a cDNA library to characterize genes expressed in haustoria of Pst.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>A total of 5,126 EST sequences of high quality were generated from haustoria of Pst, from which 287 contigs and 847 singletons were derived. Approximately 10% and 26% of the 1,134 unique sequences were homologous to proteins with known functions and hypothetical proteins, respectively. The remaining 64% of the unique sequences had no significant similarities in GenBank. Fifteen genes were predicted to be proteins secreted from Pst haustoria. Analysis of ten genes, including six secreted protein genes, using quantitative RT-PCR revealed changes in transcript levels in different developmental and infection stages of the pathogen.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>The haustorial cDNA library was useful in identifying genes of the stripe rust fungus expressed during the infection process. From the library, we identified 15 genes encoding putative secreted proteins and six genes induced during the infection process. These genes are candidates for further studies to determine their functions in wheat-Pst interactions.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20028560</PMID><DateCompleted><Year>2010</Year><Month>02</Month><Day>17</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2164</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><PubDate><Year>2009</Year><Month>Dec</Month><Day>23</Day></PubDate></JournalIssue><Title>BMC genomics</Title><ISOAbbreviation>BMC Genomics</ISOAbbreviation></Journal><ArticleTitle>Generation and analysis of expression sequence tags from haustoria of the wheat stripe rust fungus Puccinia striiformis f. sp. 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Therefore, the objective of this study was to generate a cDNA library to characterize genes expressed in haustoria of Pst.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">A total of 5,126 EST sequences of high quality were generated from haustoria of Pst, from which 287 contigs and 847 singletons were derived. Approximately 10% and 26% of the 1,134 unique sequences were homologous to proteins with known functions and hypothetical proteins, respectively. The remaining 64% of the unique sequences had no significant similarities in GenBank. Fifteen genes were predicted to be proteins secreted from Pst haustoria. Analysis of ten genes, including six secreted protein genes, using quantitative RT-PCR revealed changes in transcript levels in different developmental and infection stages of the pathogen.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The haustorial cDNA library was useful in identifying genes of the stripe rust fungus expressed during the infection process. From the library, we identified 15 genes encoding putative secreted proteins and six genes induced during the infection process. 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Hulbert</name><name sortKey="Kang, Zhensheng" sort="Kang, Zhensheng" uniqKey="Kang Z" first="Zhensheng" last="Kang">Zhensheng Kang</name><name sortKey="Wang, Xiaojie" sort="Wang, Xiaojie" uniqKey="Wang X" first="Xiaojie" last="Wang">Xiaojie Wang</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Yin, Chuntao" sort="Yin, Chuntao" uniqKey="Yin C" first="Chuntao" last="Yin">Chuntao Yin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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